02873nam a2200301 4500 ESSALUD 20260805113858.0 ta t pe ||||| |||| 00| 0 spa d BMG eng Gharahdaghi, Nima Autor 53934 Interleukin-10 autoantibodies and HLA-DRB1*01:03 in inflammatory bowel disease páginas: 2212-2222 Background: Neutralizing autoantibodies against interleukin-10 can result in a phenocopy of monogenic defects of interleukin-10 signaling in children and may be associated with inflammatory bowel disease (IBD). The allele HLA-DRB1*01:03 is the strongest genetic risk factor for ulcerative colitis. Methods: We used a cellular interleukin-10 reporter assay and a confirmatory competitive enzyme-linked immunosorbent assay to assess neutralizing interleukin-10 autoantibodies in serum samples obtained from patients with IBD in the Oxford and U.K. IBD BioResource cohorts and from persons without IBD (controls). An in vitro cytokine-release bioassay was performed in a subgroup of patients to assess interleukin-10, interleukin-23, interleukin-1β, tumor necrosis factor, and interleukin-6. We performed HLA association analysis using imputation and high-resolution sequencing. Results: Interleukin-10–neutralizing autoantibodies were detected in 173 of 4909 patients with IBD (3.5%; 95% confidence interval [CI], 3.0 to 4.1) and in none of 1006 controls (P<0.001). High anti–interleukin-10 activity in serum was associated with a reduction in detectable interleukin-10 and with an exaggerated proinflammatory cytokine response, consistent with functional neutralization of interleukin-10 signaling. Anti–interleukin-10 seropositivity was strongly associated with HLA-DRB1*01:03 on the basis of imputed data from the Oxford cohort (odds ratio, 50.0; 95% CI, 16.4 to 152.3; P=6.14×10−12) and the U.K. IBD BioResource cohort (odds ratio, 24.7; 95% CI, 14.5 to 42.1; P=6.20×10−32) and in a high-resolution sequencing analysis of data from the Oxford cohort (odds ratio, 29.5; 95% CI, 12.2 to 71.1; P=4.85×10−14). Conclusions: Neutralizing interleukin-10 autoantibodies were present in a subgroup of patients with IBD and were strongly associated with HLA-DRB1*01:03. ALERGIA 7055 ENFERMEDAD AUTOINMUNE 53665 GENETICA 1105 GASTROENTEROLOGÍA 12562 ENFERMEDAD INFLAMATORIA INTESTINAL 53935 Yeh, Pai-Jui 53936 Cerón-Gutiérrez, Lourdes 53937 Griffin, Helen 53938 Gordon, Hannah 53939 Jayamanne, Chamara 53940 22717 22641 Massachusetts NEJM Group NEJM008 The New England Journal of Medicine ESSALUD 0028-4793 ARTICULOS 2026-08-03 SQB 22776 22776